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FAST Plaque TB. They believe that this new low A total of 2000 urine samples collected from
cost assay may have widespread applicability, Mirpur. Dhaka, was examined for the presence of
especially in developing countries, due to its urinary tract infection (UTI) during November
manual format and rapid reporting of results. 2001 to January 2002 and from April to June 2002.
47.6% cases showed Escherichia coli positive.
295 MUNSHI, S.U.; ISLAM, M.N.; The overall prevalence of the UTI was higher
TABASSUM, S. (Dept. Of Virology, BSMMU, during the summer (52.40%) than in the winter
Dhaka). QUANTIFICATION OF HIV-1 RNA BY (40.40%). During the summer, 39.26% male and
NUCLEIC ACID SEQUENCE BASED 60.73% female cases were detected as E. coli
AMPLIFICATION (NASBA) ASSAY USING positive. The women of different age-groups were
ELECTROCHEMILUMINESCENCE (ECL) IN prone to E. coli infection. High prevalence of the
CLINICAL SAMPLES AND IT’S FUTURE IN UTI was found among the children of different
BANGLADESH. Bang.Med. J., 2006, 35 (2), 49- age-groups, 48.20% during the summer and
53. 20.70% during the winter. The E. coli infection in
women possess virulence associated properties.
Management of HIV infected individuals has The highest percentage of positive cases (72%)
greatly improved with the introduction of were found among the adult women (21-25 years).
quantitative viral load testing and anti-retroviral
therapy (ART). Recently viral load testing has 297 RABBI, F.J.; SHIRIN, T. (Dept. of
been introduced in Bangladesh. This study is done Clinical Immunology, National Institue of Kidney
to observe gross technical efficiency of viral load Diseases and Urology, Sher-e- Bangla Nagar,
test as an assay of choice in a laboratory of third Dhaka) & REZWAN, M.K. (Dept. of
world countries like Bangladesh .Viral load of 42 Immunization and Vaccination, WHO, Indonesia).
serum specimens (12 positive, 5 false positive for HBeAg/anti-HBe, alanine aminotransferase and
HIV-1 antibody by Enzyme Linked Immuno HBV DNA levels in HBsAg positive chronic
Sorbent Assay ; ELISA / Particle Agglutination carriers. Bang.Med. Res. Counc. Bull., 2008, 34
Test; PAT) were measured by Nucleic Acid (2), 39-43.
Sequence Based Amplification (NASBA) followed
by Electrochemiluminescence (NucliSens HIV- Serum samples from a total of 72 chronic hepatitis
QT). In the HIV positive group, the RNA levels B virus carriers were analysed by serological,
were ranged from 310 copies to 75,000 copies /ml. biochemical and molecular assays. The aim was to
In the negative sera RNA copies were below the evaluate the relationship of the serological and
lower detection limit (LDL). NASBA followed by biochemical parameters with molecular markers in
Electrochemiluminescence is good as well as order to assess the infectivity of virus. Out of 72
expensive tool for measurement of viral load in our chronic HBsAg positive earners, 28 patients were
country. As this is true for all Nucleic Acid assay HBeAg positive and anti-HBe negative, 38 patients
methods, the concern corners should consider were HBeAg negative and anti-HBe positive, only
lowering down the price of viral load testing, as it 3 patients were positive for both HBeAg and anti-
has already done for ART in third world countries; HBe and the rest 3 patients were negative for both
otherwise lowering down of transmission rate of markers. Detectable HBV DNA level was found in
HIV in this poor people group will be a great 92.86% HBsAg-positive/anti-HBe negative
barrier in future . patients along with raised alanine aminotransferase
(ALT) level (67.86%) compared with HBeAg-
296 NAHAR, S.J.; KHANUM, H. & negativc/anti-HBe positive carriers (36.84%) (p
FARHANA, R. (Dept. of Zoology, Dhaka value = 0.02) and out a total of 38 HBeAg-
University, Dhaka). OCCURRENCE OF negative/anti-HBe positive carriers, 12 (31.58%)
patients had detectable level of HBV DNA.
ESCHERICHIA COLI INFECTION AMONG Among the 14 HBeAg-negative /anti-HBe positive
THE CHILDREN OF DHAKA CITY. Bang.J. patients with elevated ALT level. 8 (57.14%) had
Zool., 2006, 34 (2), 181-186. detectable HBV DNA whereas out of 24 HBeAg-
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