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length of shoot and root, fresh and dry weights of organogenesis and somatic embryogenesis. Bang.
shoot and root per plant at early seedling stage J. Life Sci., 2006, 18 (1), 27-38.
were significantly reduced by salinity in all the 6 This paper describes in vitro clonal multiplication
varieties. Among the varieties, BARImung-2 of two varieties, Khaja and Gula of Artocarpus
showed the highest reduction in germination heterophyllus and evaluation of field performance
percentage and germination index, whereas those of regenerated plants in comparison with seedling
were lowest in BUImung-2, especially at 200 plants. For Khaja variety, MS medium
-1
-1
mM NaCl solution. the reduction of seedling supplemented with 2 mgl BA + 0.5 mgl NAA
height, fresh and dry weights of shoot and root was found to be optimum for multiple shoot
was also the highest in BARImung-2, whereas induction in shoot tip explants. On the other hand
that was the lowest in BUImung-2. Based on the in Gula variety, the best medium was MS + 1.0
-1
-1
results, out of six mungbean varieties, BUImung- mgl BA + 0.5 mgl Kn. A clump of 7-10 shoots
2 showed the highest, while BARImung-2 the was produced after two to three subcultures. For
lowest salt tolerance in terms of both absolute both the varieties, addition of 15% CW + 150
-1
and relative growth performance at the early mgl CH to the media enhanced the number of
seedling stage. shoot up to 25 per culture. For somatic
embryogenesis in Khaja variety, germinating
581.4 : BOTANY : MORPHOLOGY seeds were used as explants. Initially 90 and 75%
cultures of epicotyls and cotyledon explants,
-1
111 BHUIYAN, M.N.I. (BCSIR Laboratories, respectively produced callus in MS + 2.5 mgl
Chittagong); MOZMADER, T.I.M.A. & 2,4-D. When pieces of 0.5 g callus were
-1
-1
RAHMAN, S. (Dept. of Botany, Dhaka subcultured in MS + 3.0 mgl BA + 1.0 mgl IBA
-1
University, Dhaka). Microbial Antagonism and + 300 mgl L-glutamine somatic embryos were
Induction of Mutation in Neurospora Crassa by developed. About 55% embryos converted into
1
Crude Leaves Extract of Averrhoa carambola. L. plantlets when subcultured in MS + 1 mgl" GA 3
Bang. J. Sci. Ind. Res., 2007, 42 (4), 503-508. + 10% CW within 30-35 days. For the evaluation
of field performance all the categories of
Crude extract of leaves from Averrhoa carambola regenerated plants along with seedling-raised
.L showed significant inhibition of growth and individuals were planted in the field and observed
th
mutagenesis in Neurospora crassa. Result showed up to 5 year. At the end of fifth year height
that 1 ml extract reacted with the test organism, N. growth of regenerated Khaja, Gula and
crassa very much. During the period of 24 hours somaticembryo raised plants were 565, 522, 511
it gave only 2.4 cm linear vegetative growth of the cm, respectively and that of Khaja and Gula
mycelia. Whereas 0.5 ml and 0.25 ml extracts seedling raised plants were 542 and 518 cm,
gave less reactions (1.75 cm and 2.25 cm respectively. On the other hand, al the end of fifth
respectively). The linear growth decreased with year the base diameters of the plants of same
the increased of the concentration of the extracts. categories were 22.83, 22.15, 20.58, 23.25 and
Conidia of Ema (5297) of N. crassa were treated 23.27 cm, respectively. In the present study within
for 3-4 hours separately in 100% and 50% five years no abnormality in growth and
concentration of the extracts. 100% extracts morphology was observed.
produced 4 types mutants namely ropy, albino,
dirty and buff. 50% extracts produced 4 types of 113 KHATUN, R.; ISLAM, A.S.
mutants namely- plug, vigorous, pig-mented and (Biological Research Division, BCSIR
conidial band. Laboratories, Dhaka) & MAJID, F.Z. (Dept. of
Botany, Dhaka University, Dhaka). Study of
112 DEBNATH, R.K. ; HASSAN, A.K.M.S. Water Hyacinth of Bangladesh IV Comparative
& ROY, S.K. (Dept. of Botany, Jahangirnagar Study of Pollen Grains and Development of Seed
University, Savar, Dhaka). Evaluation of field in Both Long and Short Petioed Forms of
performance of jackfruit (Artocarpus Eichhornia Crassipes (Mart.) Solms-Laubach.
heterophyllus Lam.) regenerated through Bang. J. Sci. Ind. Res., 2006, 41 (3 & 4), 189-202.
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