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cfu/ml, the culturability extended for more than 20 University of Dhaka, Dhaka); HAQ, J.A. (Dept.
days.In all cases, the strain became nonculturable of Microbiology, BIRDEM, Dhaka) & KHAN,
after 7 to more than 20 days depending on initial M.R. (Dept. of Botany, Dhaka University, Dhaka).
inoculum density and type of water but remained Bancroftian Microfilariae in Human Blood
viable which was determined by fluorescence Samples from Rangpur, Bangladesh. Bang. J.
microscopy using DAPI staining and the Shigella Microbiol., 2005, 22 (1) 64-67.
spp. specific gene amplification by PCR.
Uttam Union, Rangpur, is endemic for the
099 UDDIN, M.N.; KHAN, A.R. (Dept. of mosquito borne disease, lymphatic filariasis,
Microbilogy, University of Dhaka, Dhaka); caused by nematode parasite dwelling in the
ABDULLAH-AL-MAHIN & BEGUM, R. lymphatic system. To detect the young parasite,
(Microbiology & Industrial Irradiation Division, microfilaria (mf), a total of 185 venous blood
Institute of Food & Radiation Biology (IFRB), (nocturnal) samples were screened. Among the
Bangladesh Atomic Energy Commission (BAEC), volunteers, 117 were males and 68 were females.
Dhaka). Assessnent of Microbiological Quality of Mf positive individuals were found to be 28 among
Some Fast Foods in Relation to Safety and which, 23 were males and 5 were females. Stained
Hygiene. Bang. J. Microbiol., 2005, 22 (1), 72-75. blood smears and sediments of osmolysed blood
were studied under microscope. Maximum mf
The microbiological quality of ten different count was 40 per 100 µl of blood for stained
varieties of fast foods collected from different smears and total number of mf positive individuals
shops in Dhaka city was assessed. On the basis of was 18. After osmolytic treatment (concentration
total viable bacterial count (TVBC), total coliform technique), maximum number of mf was 264 per
count (TCC) total faecal coliform count (TFCC) 100 µl of blood and total mf positive individuals
and total Staphylococcus count (TSC), respectively were 24. Thus the osmolytic procedure was found
10, 60, 10 and 90% fast foods collected from high- to be more effective to demonstrate mf in blood.
class shops were found be unsatisfactory. No food The length of the microfilariae (mfs) obtained
items were found to be unsatisfactory on the basis ranged from 230-360µm. Morphology of the
of total Aeromonas count (TAC) or total fungal microfilarae revealed the presence of bancroftian
count (TFC).The amount of unsatisfactory filariasis in the study area and this was supported
samples, obtained from middle-class shops, on the also by immunochormatographic test (ICT).
basis of TVBC, TCC, TFCC, TSC and TAC were
40, 80, 60, 100 and 10% respectively. The most 101 HAQUE, M.S. ; KARIM, M.R. (Dept.
alarming results were obtained from the food items of Biochemistry and Molecular Biology, Rajshahi
collected from lower class shops where 100% food University, Rajshahi) & SHIMAZU, T. (Dept. of
samples were found unsatisfactory on the basis of Medical Biochemistry, Ehime University School of
TVBC, TCC, TFCC and TSC. On the basis of Medicine, Japan). Peripheral glucose uptake after
TAC and TFC, 10 and 30% samples respectively injection of leptin into the ventromedial
were found unsatisfactory. Some food items were hypothalamus of anesthetized rat. Bang. J. Life
found to be contaminated with pathogenic Sci., 2006, 18 (1), 131-138.
microorganisms regardless of the sample area. The
3
safety and hygienic status of fast foods of high- The rate constant of 2-[ H]DG uptake (Ki) was
class restaurants were found to be comparatively calculated in the heart, spleen, white adipose tissue
better than those of middle-class and lower-class (WAT) and brown adipose tissue (BAT) of
shops. anesthetized rats in response to microinjection of
leptin into the VMH. Total time of leptin
577.1 : BIOLOGY : BIOCHEMISTRY stimulation was 75 minute. In the heart and spleen,
-l
the Ki (min ) values were 171.6 ± 20.0 and 39.6 ±
100 ELAHI, M.; YASMIN, L. SULTANA, 2.5 for the leptin-tread group and that of the
S.; NAVID, A.F.; (Dept. of Biochemistry & control rats were 162.9 ± 37.9 and 32.5 ± 2.6
Melecular Biology, Faculty of Biological Sciences, respectively. Similarly, in the epididymal WAT,
-1
the Ki (mi ) values were 2.8 ± 0.59 and 2.4 ± 0.45
27
