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FROM STORED COFFEE BERRIES. J. Bio-Sci., Group, Dhaka). Optimization of cultural
2006, 14, 39-41. parameters under solid state fermentation for the
production of xylanase by Thermomyces
A new species of predatory mite of the family lanuginosus. Bang. J. Life Sci., 2006, 18 (1), 85-
Phytoseiidae viz., Amblyseius (Amblyseius) coffeae 90.
sp. nov. is described from stored coffee berries.
The thermophilic fungus Thermomyces
576.8 : BIOLOGY : MICROBIOLOGY, lanuginosus was studied under solid state
BACTERIOLOGY & PARASITOLOGY fermentation (SSF) using wheat bran for the
production of xylanase. The optimum temperature
and moisture content for xylanase production was
092 AKHTER, M.Z.; HUSSAIN, A. found to be 55°C and 80% respectively.The
KHAN, S.N. (Dept. of Microbilogy, University highest enzyme activity was observed at pH 6.5
of Dhaka, Dhaka).Plasmid-Mediated Multi-Drug and was exhibited after 7 days of incubation. For
Resistance of Escherichia coli Isolated from increased xylanase production mineral solution
Urinary Tract Infections: A Preliminary Study. was not necessary and among the different natural
Bang. J. Microbiol., 2005, 22 (1), 55-58. lignocellulosic carbon sources, wheat bran was
found lo be most effective.
The present study was a preliminary investigation
of plasmid-mediated drug resistance of 094 ALAM, M.J. (Dept. of Environmental
Escherichia coli isolates from patients with urinary Hygine, Faculty of Pharmaceutical Science,
tract infection (IJTI). The techniques used were Okayama University, Okayama, Japan). Low
antibiogram, extraction of plasmid DNA and Abundance of Vibrio cholerae with ctx Gene in the
curing of plasmids. Among the 50 isolates studied, Seto-Inland Sea, Japan. Bang. J. Microbiol., 2005,
41 were found to be multi-drug resistant exhibiting 22 (1), 47-49.
resistance to eight out of ten antibiotics used,
namely, ampicillin, amoxycillin, ceftazidime, Toxigenic Vibrio cholerae is a pathogenic
ceftriaxone, cephalexin, cloxacillin, tetracycline bacterium, naturally occurring in the estuarine
and gentamicin. All of these isolates were sensitive and marine environments throughout the
to imipenem and nitrofurantoin. plasmid DNA world. The incidence of this organism in an
bands of various sizes were observed in 34 aquatic environment varies depend upon many
isolates, which ranged from 1 to 16 kbp. Twenty- ecologic factors. Seawater and organic
five of these isolates showed multiple plasmid materials (live and/or dead plants and animals)
bands, many of which had bands with similar sizes. were collected during cool and warm weather
The most common and prominent band was seasons from 5 areas of the Seto-lnland Sea,
observed in the 16-kbp position. plasmid curing and analysed to detect the toxigenic V.
experiments were performed with three different cholerae, defined as those possessing the
conenrations of SDS (5, 8 and 10%), acridine cholera toxin gene (ctx) by the polymerase
orange (20, 30 and 50 µ.g/ml) and ethidium chain reaction (PCR), using isolated DNA
bromide (50, 70 and 100 µg/m1) as curing agents. from enrichment culture of the samples. About
Acridine orange (50 µg/ml) and ethidium bromide 8% samples were positive for toxigenic V.
(100 µg/ml) were found to be effective for plasmid cholerae with densities of 3 to 11 cells/100 ml
curing. Cured cells became ampicillin sensitive water or 10 g organic samples by most-
and showed no plasmid bands, which proved that probable-number (MPN)-PCR technique. The
the ampicillin resistance property of the multi-drug results of this study demonstrated the low
resistance (MDR) E. coli isolates were plasmid- number of the toxigenic V. cholerae in an
mediated. important seafood production area.The finding
indicates the potential infection risk associated
093 AKHTAR, N.; HOSSAIN, F. (Dept. of with seafood harvested from this subtropical
Botany, Jahangirnagar University, Savar, Dhaka) aquatic environment.
& MOHIUDDIN, G. (International Study
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