Page 33 - PARVEEN, S

095 HAQUE, M.M.; SHEIKH, M.A.; of Environmental Science and Management,
KHANAM, M. (Dept. of Microbiology, Dhaka
University, Dhaka) & NAHAR, N. (Badrunnessa
Govt. Girl's College, Dhaka). α-Amylase
Production from Streptomyces griseoloalbus and
Some Factors Influencing the Enzyme Activity.
Bang. J. Microbiol., 2005, 22 (1), 76-78.
Three indigenous Streptomyces species, namely, S.
microflavus, S. griseoloalbus and S, kunmingensis,
were selected for their α-amylase production.
Among the three species studied, S. griseoloalbus
(S-21) showed highest α-amylase activity.The
effect of pH and temperature on α-amylase activity
and stability was determined. The pH and
temperature optima of α-amylase from S.
0
griseoloalbits were 7.0 and 45 C, respectively. The
0
enzyme was stable at temperature up to 50 C, and
above that the activity sharply reduced. The
enzyme was most stable at pH 7.0.
096 KHANUM, H.; KABIR, R.; DAY,
R.R. ; FARHANA R. (Parasitology Branch,
Dept. of Zoology, Dhaka University, Dhaka) &
MASUM, M.A. (Parasitology Dept. Institute of
Epidemiology, Disease Control and Research).
COMPARISON OF DIRECT AGGLUTINATION
TEST AND ALDEHYDE TEST IN SERODIAGNOSIS
OF VISCERAL LEISHMANIASIS. Bang. J.
Zool., 2008, 36 (1), 93-99.
Direct Agglutination Test (DAT) and Aldehyde
Test (AT) were assessed and compared in
serodiagnosis of the visceral leishmaniasis (VL) of
400 suspected cases from different parts of
Bangladesh. Trypsin treatment of Leishmania
donovani promastigote antigen proved to be
indespensible in the direct agglutination test for the
diagnosis of VL. Significant titers were found in
220 (55%) sera by the DAT and 260 (65%) sera
formed anti-leishmania antibodies by the AT. A
titer of > 1: 3200 was considered indicative for
visceral leishmaniasis. The sensitivity and
specificity of the DAT were 100% and 98.6%.
respectively, whereas, the AT showed 84%
sensitivity and 64.3% specificity.
097 MAHMUDA, S. (Dept. of Botany,
Jahangirnagar University, Savar, Dhaka);
DEBNATH, R.K. & HAQUE, M.M. (School
26
Independent University Bangladesh (IUB),
Dhaka). Determination and acquisition of anti-
microbial compounds secreted during the
interaction between Nostoc sp. and other
microorganisms. Bang. J. Life Sci., 2008, 20 (1),
51-56.
In a survey of microbes from field grown terrestrial
cyanobacterium, Nostoc sp, was collected from
Dhaka city. Fifteen microbes were isolated with
cyanobacterial sample and those were separately
subjected to shaking culture. The culture broths
were then assayed for anti-microbial activities
using Staphylococcus aureus as a test organism.
Among them three isolates showed strong anti-
microbial activity against S. aureus. A dual culture
for Nostoc sp. and Paenibacillus glynilyticus was
performed in microcosm to elucidate the anti-
bacterial compounds secreted during the
interaction between those two tests organisms. The
bioactive compound released in the medium of
dual culture were isolated and identified as anti-
microbial compound, which showed pronounced
toxicity against S. aureus.
098 SULTANA, M.; RAHMAN, M.Z.;
KHAN, S.I. (Dept. of Microbiology, University
of Dhaka, Dhaka) & BIRKELAND, N.K. (Dept.
of Microbiology, Bergen University, Norway).
Survival of Shigella Flexneri Cells in Laboratory
Microcosms. Bang. J. Microbiol., 2005, 22 (1), 19-
22.
The survival of Shigella flexneri was studied in
laboratory microcosms to assess their culturability.
Laboratory microcosms were set up using water
from tap, pond and lake. In each series, three
6 7 8
different densities, e.g., 10 , 10 and 10 cfu/ml
were used. The findings of the present study
showed that survivility of Shigella flexneri
increases with the increase of inoculum density.
Survival potential of the cells was higher in case of
tap water followed by pond and lake water. With
6
the initial inoculum density of 10 cfu/ml, the
strain became nonculturable after 7, 8 and 10 days
in lake, pond and lap water respectively. When the
7
initial inoculum was 10 cfu/ml, culturability of the
strain increased up to 10 days in lake and pond
waters whereas it was 12 days with tap water.
However with the highest inoculum level of 10
8