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form an n-hexane extract of the stem bark of potential (+155mV for Fe TMPyP and +60mV for
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Eclipta Prostrata. The structures of the isolated Fe TSPP at pH 2.0) and the onset of emission was
compounds were confirmed by extensive found pH dependent.The redox potential calcutated
spectroscopic studies, notably high field NMR as the average values of upward and downward
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and MS. The 13 C NMR data of the parent emission for Fe TSPP shifted towards cathodic
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saponins 1 and 2 are reported here the first potential compared to that of Fe TMPyP by more
time. than 65mV. Therefore, two pKa values were
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estimated as 5.7 and 11.7 for Fe TMPyP and that
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268 RAHMAN, S.; CHOWDHURY, A.A.; for Fe TSPP were 6.5 and 12.3 reflecting the
HASNAT, A. (Faculty of Pharmacy, Dhaka charge effect on the redox potentials.
University, Dhaka) & KARIM, P. (Dept. of
Pharmacy, Asia Pacific University, Dhaka). Effect 270 RAZIB, B.M.M.A.; ULLAH, M.A.;
of Manganese on the Activity of Antibiotic AZAD, M. A.K.; SULTANA, R.; YASMIN, H.
Against Microorganisms. Dhaka Univ. J. Pharm. & HASNAT, A. (Dept. of Clinical Pharmacy and
Sci., 2005, 4 (1), 23-26. Pharmacology, Dhaka University, Dhaka).
Validation and Application of a Modified RP-
Manganese is an important element in biological HPLC Method for the Quantification of
system. It is essential for enzymatic activity, Desloratadine in Pharmaceutical Dosage Forms.
maintaining three-dimensional structure of protein, Dhaka Univ. J. Phar. Sci., 2006, 5 (1-2), 1-4.
for the synthesis of nucleic acid and protein etc.
Deficiency of manganese causes different life The purpose of the study was to develop a
threatening diseases. Because of this, the optimum simple, sensitive and rapid RP-HPLC method
level of manganese must be maintained in all for the determination of desloratadine in
biological system. This experiment, therefore, was marketed products. Chromatographic
designed to evaluate the effect of manganese on determination was performed in a reverse phase
the activity of different antibiotics (ciprofloxacin, C 18 column (250 mm x 3.3 mm I.D., 5µm
cephradine, amoxicillin, gentamycin, tetracycline, particle size) using a mixture of acetonitrile : n-
cloxacillin, nalidixic acid, ceftriaxone, pentane sulphonic acid sodium salt
metronidazole and carbenicillin) against different monohydrate, adjusted to pH 3.0± 0.05 with
microorganisms. It has been observed that phosphoric acid (60:40 v/v) as mobile phase and
antimicrobial activity of an antibiotic increased delivered at a flow rate of 1 ml/min. The UV
significantly with concomitant use of manganese detection was set at 254 nm. The calibration
salt ranging from 600-6000 ng per antibiotic disc range was from 2.0 to 40 µg/ml. The method
(P < 0.05). It is revealed from the experiment that was validated in term of validated in trem of
2
manganese increases the activity of antibiotic linearity (r >0.98, RSD=1.958%) precision
against bacterial strains. (RSD=3.757%) and accuracy
(deviation<2.653%, RSD< 2.203%). The limit
269 RANA, M.S. (Dept. of Pharmacy, of quantification was 2 µg/ml and the limit of
Jahangirnagar University, Savar, Dhaka). Study on detection was 0.1 µg/ml. The lienear ranges of
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electrochemical reduction of Fe TMPyP and desloratadine were 20.23 ± 0.368 µg/ml and
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Fe TSPP by luminol chemiluminescence. Bang. J. 6.545 ± 0.0495 µg/ml in tablet (potency =
Life Sci., 2006, 18 (1), 1-9. 99.175 ±0.718 %) and syrup (potency = 101.15
± 1.838 %) respectively. The potency of
Formation of ferryl species induced by desloratadine in marketed products was
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electrochemical reduction of Fe TMPyP and determined by this method with acceptable
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Fe TSPP was examined by measuring luminol precision and reproducibility.
chemiluminescence using flow-injection method.
Ferryl is widely accepted to be the responsible 271 REZA, A.F.G.M. (Dept. of Chemistry,
species for stimulating emission in hydrogen National University, Gazipur); KHAN, M.W.
peroxide / iron porphyrin / luminol system. (Dept. of Chemistry, BUET, Dhaka); RAHMAN,
Chemiluminescence observed below the reduction M.S.; HASAN, C.M. (Dept. of Pharmaceutical
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