Page 85 - PARVEEN, S
P. 85
such as, pH and sedimentation of the tested syrups 24-kDa protein following induction of transformed
were found satisfactory. cells confirmed the expression of the cloned gene,
human elF4E.
263 RAHIM, M. A. & RANA, M.S. (Dept. of
Pharmacy, Jahangirnagar University, Savar, 265 RAHMAN, M.M,; KHONDKAR, P.
Dhaka). Study on some physical characteristics of (Dept. of Pharmacy, Rajshahi University,
commercially available enteric-coated NSAIDS. Rajshahi) & GRAY, A.I. (Dept. of Pharmaceutical
Bang. J. Life Sci., 2006, 18 (2), 71-75. Sciences, Strathclyde University, Scotland, UK.).
Terpenoids from Atylosia Scarabaeoides and their
Four commercial brands of enteric-coated antimicrobial activity. Dhaka Univ. J. Pharm. Sci.,
NSAIDs, two of aspirin & diclofenac Na each, 2005, 4 (2), 141-144.
were evaluated to examine the hardness and
disintegration profile. Furthermore, it was tried to Three terpenes-caryophyllene-4,5-oxide, α-amyrin
find out the correlation, if any, between these two and β-amyrin were isolated from the petroleum
parameters. The results show that although the ether extract of Atylosia scarabaeoides.The
sample of three different batches under structures of the compounds were confirmed by a
investigation had disintegrated within the BP time series of 1D and 2D NMR and MS analyses. The
limit, the enteric coating on several tablets of four minimum inhibitory concentration (MIC) of
batches, one of aspirin & three of diclofenac caryophyllene-4,5-oxide was found to be 50-200
sodium had failed to provide necessary protection. µg/ml.
Again, all the three batches of one brand of aspirin
disintegrating in fairly distant regions showed 266 RAHMAN, M.S.; CHOWDHURY, R.;
prominent inter batch variation. The hardness BEGUM, B.; RAHMAN, K. M. & RASHID, M.
profile was found to be satisfactory in every batch. A. (Phytochemical Research Laboratory, Dept. of
Pharmaceutical Chemistry, Dhaka University,
264 RAHMAN, M. & KARIM, M.M. (Dept. of Dhaka). Phytochemical Studies of Amoora
Microbiology, Dhaka University, Dhaka). cucullata. Dhaka Univ. J. Pharm. Sci., 2005, 4
Heterologous Expression of a Human Cap-Binding (1), 73-75.
Protein elF4E in Escherichia Coli. Dhaka. Univ. J.
Pharm. Sci., 2006, 5 (1-2), 59-62. Five compounds were isolated from a hexane
extract of the stem bark of Amoora cucullata. The
A crucial step in the initiation of cap dependent structures of the isolated compounds were
translation of eukaryotic mRNA is the interaction established as fridelin (1), stigmasterol, β-
of the mRNA cap-binding protein, elF4E, with sitosterol, betulinic acid (2) and caffeic acid (3) by
elF4G, two integral components of the mRNA cap- extensive spectroscopic studies, including high
binding complex. The present study was aimed to field NMR and MS analyses. This is the first report
express human elF4E at high level in E. Coli. For of occurrence of these compounds from A.
hig-level expression, the E. coli cells were first cucullata.
transformed with an expression vector that
contains the cloned gene, human elF4E. Following 267 RAHMAN, M.S; CHOWDHURY, R.;
transformation, the recombinant plasmid was HASAN, C.M. (Phytochemical Research
purified stepwise digestion of the recombinant Laboratory, Dept. of Pharmaceutical Chemistry,
plasmid revealed that the plasmid was harboring an Dhaka University, Dhaka) & RASHID, M. A.
insert, equal to the length of the elF4E gene. As the (Centre for Biomedical Research, Dhaka
vector contained a heat-inducible promoter, high- University, Dhaka). Oleanane glycosides from
level expression of human elF4E was carried out Eclipta Prostrata. Dhaka Univ. J. Pharm. Sci.,
0
by heat induction at 42 C. Expression of 2005, 4 (2), 107-111.
recombinant protein was observed by comparing
the cell extracts collected before and after heat Two oleanane-type glycosides eclalbasaponin
induction and their subsequent analysis on a II (1) and eclalbasaponin I (2) along the
denatured polyacrylamide gel. The appearance of a ubiquitous steriod, stigmasterol were isolated
78
