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therapy of Hepatitis B virus (HBV) infection. The imparting agents. It is evident from the result that
researchers have analyzed the quantity of HBV- lag time increases with the increase in percentage
DNA during lamivudine therapy and investigated of viscosity imparting agent. There is less or no
the relationship of lamivudine resistance to effect of change of concentration of acacia on the
mutation type. Ninety-one hepatitis B patients lag time. After lag time drug release from the
were enrolled in the study where Real Time suppositories showed a linear fashion. It was found
Polymerase Chain Reaction (PCR) did estimation that the release rate decreases when dissolution
of HBV DNA and mutation was analyzed by medium contains high percentage of Xanthan gum
sequence detection via PCR, HBV, DNA was and also sodium carboxy methyl cellulose.
detected in the serum of 96.7% (88/91) Patients However in case of incorporation of HPMC into
with mean viral load ranging from 1×10 to 1×16 the dissolution medium, release rate decreased up
More than 80% Patients responded to and 17.3% to 0.2% HPMC, but with 0.3% HPMC the release
patients showed resistance to lamivudine therapy. rate increased. Inclusion of different perccntage of
All lamivudine resistant patients had HBV YMDD acacia into the dissolution medium has not
mutation of eithe 180M/M 204V or rtL180/M2041 significantly changed the release of acetaminophen
types. PCR based analysis of HBV DNA and from suppositories.
sequence basd mutation detection can be
practically, feasible approach in Bangladesh to 223 AKBOR, M.M.; SULTANA, R.;
monitor hepatitis B patients under lamivudine ULLAH, A.; HASNAT, A.(Dept. of Clinical
therapy. Pharmacy and Pharmacology, Dhaka University,
Dhaka); AZAD, M.A.K.;(Dept. of
222 AKANDA, M.K.; ISLAM, S.M.A.; Pharmaceutical Technology, Dhaka University,
CHOWDHURY, J.A. (Dept. of Pharmacy, Asia Dhaka) & LATIF, A.H.M.M. (Institute of
Pacific Univsrsity, Dhaka) & REZA, M.S. Statistical Reseasch and Training, Dhaka
(Faculty of Pharmacy, Dhaka University, Dhaka). University, Dhaka). IN VITRO-IN VIVO
EFFECT OF VISCOSITY IMPARTING AGENTS CORRELATION (IVIVC) OF IMMEDIATE
ON IN VITRO DRUG RELEASE FROM PEG RELEASE (IR) LEVOFLOXACIN TABLET.
BASED SUPPOSITORIES. Dhaka Univ.J. Pharm. Dhaka Univ. J. Pharm. Sci., 2007, 6(2), 113-119.
Sci., 2005, 4 (1), 1-5.
The aim of this study was to examine the in vitro -
Acetaminophen loaded suppositories were in vivo correlation (1VIVC) of two immediate
prepared and the effects of viscosity imparting release formulations of levofloxacin 250 mg film
agents on drug release were investigated. coated tablet. In vitro release data were obtained
Suppositories containing 125 mg of acetaminophen for each formulation by using the USP apparatus
were prepared by fusion method using PEG 4000 II, in 0.IN HCI of pH 2.0. Twelve healthy subjects
and PEG 1500 as hydrophilic base. In vitro were randomly chosen for in vivo study. Blood
dissolution studies were carried out by a thermal samples were collected over 24 hour period and
shaker with a shaking speed of 90 rpm at a analysed by validated HPLC method with UV
0
temperature of 37 ± 0.5 C in phosphate buffer of detection to establish a correlation between in vitro
pH 6.8. The effect of viscosity imparting agents on release and in vivo absorption. f 2 and f 1 were
the drug release into phosphate buffer were determined for the time intervals of 5, 10,
investigated by adding 0.1, 0.2 and 0,3% Xanthan 15,20,25, 30, 45 and 60 minutes and the obtained
gum, sodium carboxy methyl cellulose, acacia values were 65.72, 77.38, 61.93, 53.75, 55.36,
hydroxyl propyl methyl cellulose 15 cps and 50 58.59, 60.23 and 61.16 percent for f 2 and 11.00,
cps.In vitro release data showed that drug release 4.35, 8.30, 12.15, 5.59, 1.82, 0.06 and 0.11 percent
was linear in phosphate buffer. After incorporation for f 1 at respective time intervals. Mean dissolution
of viscosity imparting agent phosphate bufter a times (MDT) for local and reference products were
biphasis drug release profile i.e. initial lag phase obtained at 7.69 and 9.55 minutes respectively.
followed by linear phase was observed. Lag time The values of mean absorption time (MAT) were
depends on nature and concentration of viscosity obtained at 8.27 and 27.96 minutes for local and
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