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explants produced shoots. The average number of
445 ROY, P.K. (Institute of Food and Radiation shoot formed per explant was 4.0 ± 1.20 and the
Biology, Atomic Energy Research Establishment, average shoot length of 4.50 ± 0.80 cm were
Dhaka). Clonal Propagation of Selected Elite observed in this medium also after 30 days of
Citrus Tree, Citrus Grandis Osbeck. through in culture. Shoots rooted well when they were excised
vitro Culture. Bang. J. Life Sci., 2008, 20 (1), 123- individually and implanted on half strength MS
130. medium supplemented with 1.0 mg/l IBA, in
which 80% of the shoot induced roots. The average
A protocol for direct regeneration of the selected number of root per shoot was 5.0 ± 1.0 and the
elite citrus tree, Citrus grandis is described. Two average root length of 6.0 ± 1.0 cm was recorded
types of explants, shoot tips and nodal segments in this medium culture after 30 days.
were used in the experiment. A high frequency
of shoot regeneration was achieved on MS+1.0 447 ROY, P.K. & KABIR, M.H. (Institute of
–1
–1
mgl BAP+0.25 mgl NAA. With repeated Food and Radiation Biology, Atomic Energy
–1
subculture and addition of 100 mgl casein Research Establishment, Savar, Dhaka).
hydrolysate to the medium enhanced the number Micropropagation of Apple, Malus Domestica
of shoots per culture and incorporation of 100 Borkh through in vitro Culture. Bang. J. Life Sci.,
–1
mgl urea to the medium increased the length of 2007, 19 (1), 107-113.
shoots. Shoots rooted well in half-strength MS
–1
supplemented with 1.5 mgl 1BA, within 12-15 For multiple shoot regeneration, shoot tips and
days. Well-rooted plantlets were transferred to nodal segments of in vitro grown seedlings of
earthen pots containing soil, sand and compost apple were used as explants. Multiple shoot
(2:1:1) for hardening. During hardening 85% induction was found best in MS medium
plantlets survived, which were subsequently supplemented with 1.5 mg/l BAP and 0.5 mg/1
transferred to the experimental field. NAA. After three weeks when they were
446 ROY, P.K. & KABIR, M.H. (Plant subcultured in the same nutrient medium, the
Biotechnology and Genetic Engineering Division, number of shoots derived from nodal explant and
Institute of Food and Radiation Biology, Atomic shoot tip explant were increased up to 20 and 14
Energy Research Establishment, Savar, Dhaka). In respectively, per culture. The addition of 100mg/l
vitro propagation of sweet orange (Citrus sinensis) urea in the medium enhanced the number of shoots
through plumule and cotyledon culture. Bang. J. per culture and incorporation of 100 mg/l CH and
Life Sci., 2006, 18 (2), 107-112. 10% (v/v) CM to the medium increased the length
of shoots. For the best rooting, well developed
A high frequency plant regeneration in sweet shoots were excised and implanted individually
orange (Citrus sinensis ) was obtained on MS onto the rooting medium containing half-strength
medium supplemented with 1.0 mg/1 BA + 0.25 MS fortified with l.0mg/l 1BA, in which 95%
mg/l NAA using plumule and cotyledon explants. rooting was achieved within four weeks.
About 80% of the plumule explants produced Regenerated plantlets were successfully
shoots. The average number of shoot per explant acclimatized and established in soil. About 85%
was 9.0 ± 2.60 and the average shoot length of plantlets survived under open field conditions.
5.50 ± 1.0 cm was obtained after 30 days of
culture. On the other hand, 50% of the cotyledon
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