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gluten was mainly due to the deamidation induced
388 RAHMAN, M.M. (Dept. of Applied
Nutrition & Food Technology, Islamic University,
Kushtia); SANA, N.K.; KHAN, M.M.H;
SARKAR, B.C.; KUMAR, S.R. (Dept. of
Biochemistry & Molecular Biology, Rajshahi
University, Rajshahi) & HUQUE, E.M. (Dept. of
Chemistry, Rajshahi University, Rajshahi). Partial
Purification of Deamidase from Germinating
wheat (Triticum Aestivum L.) Seeds and Its
Application for the Improvement of Functional
Properties of Wheat Gluten. J. Bio-Sci., 2007, 15,
89-98.
Enzyme with deamidase activity was found in the
germinating wheat seeds. The activity appeared in
the seeds after 24 hours of germination and
reached its maximum value alter 48 hours and then
declined rapidly. Protein deamidase from
germinating wheat seeds was further purified by
salting out with phosphate salts and by subsequent
chromatography on gel filtration and DEAE-
cellulose column chromatography. Polyacrylamide
slab gel electrophoresis showed the purified
enzyme to be homogeneous. The molecular weight
was determined to be 59 kDa by gel filtration and
60 kDa, by SDS-Polyacrylamide slab gel
electrophoresis (SDS-PAGE). The optimum pH
and temperature of the purified enrzyme were
found to be 6.9 and 32°C, respectively. The
functional properties of wheat gluten were
investigated after treating with it purified
deamidaso enzyme from germinating wheat seeds
at pH 6.9 at 32°C. The functional properties such
as solubility, emulsification and foam formation of
deamidase treated gluten were improved greatly as
compared to that of native gluten. The solubility of
the deamidase treated gluten was remarkably high
in the pH range of 6 to 8, in which the native
gluten was insoluble. It was apparent that the
improvement of functional properties of wheat
123
by wheat deamidase at pH 6.9 and 32°C.
389 TARIQUE, M.H. (Dept. of Agronomy and
Agricultural Extension, Rajshahi University,
Rajshahi) & PAUL, N.K. (Dept. of Botany,
Rajshahi University, Rajshahi). Effects of row
ratios in wheat-lentil intercropping system. Bang.
J. Life Sci., 2008, 20 (2), 53-57.
An experiment was conducted to assess the effects
of row ratios between wheat and lentil on the yield
and yield contributing characters as well as
economic performance of intercropping system.
The experiment was laid out in a randomized
complete block design with 3 replications. The
wheat cultivar "Gaurob" and lentil cultivar "BARI
moshur 4" were used. The highest values of yield
and yield contributing characters for both wheat
and lentil were obtained from sole cropping. Land
equivalent ratio (LER) increased from 52 to 87%
over sole crop. The highest wheat equivalent yield
(6.42 t/ha) was recorded from one row wheat
alternated with two rows lentil. This combination
also gave the highest gross return, net return and
benefit cost ratio.
633.18 : AGRICULTURE : RICE
390 ALAM, M.J.; SHAH, S.B.; ISLAM,
M.L. (Bangladesh Fisheries Research
Institute, Khulna) & TUONG, T.P. (Crop,
Soil and Water Sciences Division, International
Rice Research Institute, The Philippines).
Integrated Aquaculture with Paddy in Fresh
and Saline Water Interface of South-West
Bangladesh. Bang. J. Zool., 2007, 35 (2), 381-
389.
An experiment was conducted to study the
potentaily of integating Genetically Improved
Farmed Tilapia (GIFT) and prawn