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yield/ha November 01 gave the highest yield the best for callusing and NAA-0.5 mg 1 + BAP-
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followed by November 10. In the sowing of 1.0 mg 1 was better for shoot formation.
November 01, 10 and 20, lentil could escape the
infection of Stemphylium blight. The yield was 402 ISLAM, M.S.; AKHTER, F.; LAZ, R. &
maximum in the early sown seeds of lentil. PARWEEN, S. (Dept. of Zoology, Rajshahi
University, Rajshahi). Oviposition Preference of
401 ISLAM, M.M.; KABIR, M.Y.; PERVIN, Callosobruchus Maculatus (F.) to Common Pulses
T.; KHAN, H.I.; BHUIYAN, M.R.K. (Agro- and Potentiality of Triflumuron as Their
technology Discipline, Khulna University, Khulna). Protectant. J. Bio-Sci., 2007, 15, 83-88.
One-step Method of Plantlet Regeneration in
Mungbean (vigna radiata I. Wilczek). South Asian Seeds of black grams, lentils, Bengal grams and
J. Agric., 2007, 2 (1 & 2), 11-14. green peas were soaked separately in aqueous
solutions of Triflumuron at doses of 0.0 (control),
The present study was conducted in the Laboratory 0.5, 1.0, 1.5 and 2.0 ppm. Three day-old adults of
of Plant Tissue Culture of Agrotechnology Callosobruchus maculatus (F.) (Coleoptera:
Discipline, Khulna University, Khulna, during the Bruchidae) were allowed to oviposit on air-dried,
period from October 2006 to February 2007, with a treated or untreated pulses of each type and dose in
view to study the performance of selected 'no-choice' and 'free-choice' tests. Maximum
mungbean varieties and the effect of growth oviposition occurred in Bengal grams
hormones on callus induction and organogenesis in (61.00+_0.25) and green peas (33.67±0.54) in 'no-
a one step-method. Cotyledons of young seedlings choice' and 'free-choice' tests, respectively. The
of three mungbean varieties viz. BARI Mung4, beetles avoided egg laying on lentils. Maximum
BARI Mung54 and BARI Mung6 were cultured on egg-hatching occurred on black grams (>90%) in
MS medium supplemented with 2,4-D + BAP, both tests. Minimum developmental time was
NAA + BAP and 2,4-D + NAA + BAP. The required in the Bengal grams (30+0.5 days in 'no-
cultured cotyledons first formed callus and then choice' test and 31.33+1.23 days in 'free-choice'
regenerated into shoots or into roots without test). No adults emerged from green peas, while
transferring the calli onto regeneration medium. 54.79% and 86.15% emergence were recorded
Profuse callusing was observed in all the varieties from untreated black grams in 'no-choice' and 'free-
on all the media combinations. But the medium choice' tests, respectively. Triflumuron reduced
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containing 2,4-D- 0.5 mg l + NAA- 0.5 mg l + egg-laying significantly in green peas, where
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BAP- 1.0 mgt showed high efficiency of hatchability also reduced significantly to 35.99% at
callusing for all the studied varieties. Maximum 2 ppm in 'no-choice' test. Percentage of hatching
frequency of shoot formation was noticed among decreased in all pulses with the increasing doses of
the calli of the variety BARI Mung4, on the triflumuron. No adults emerged from the treated
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medium containing NAA-0.5 mgt + BAP- 1.0 mg green peas in any test, and at 2 ppm the adult
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l . As a whole the variety BARI Mung4 was found emergence declined to <50% in all pulses.
most efficient both for callusing and shoot
formation in comparison with other two varieties 403 KHAN, M.M.H. (Dept. of Entomology,
and among the hormone combinations 2,4-D-0.5 Patuakhali Science and Technology University,
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mg l + NAA-0.5 mg 1 + BAP- 1.0 mg 1 was Patuakhali); MANNAN, M.A. (Dept. of Agroremy,
Patuakhali Science and Technology University,
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