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9.00 mg/kg, D 4-12.00 mg/kg and D 5-15.00 mg/kg) mg/kg body weight and at that doses the
were affected equally (100%) in egg release. In the fertilization rate was 80.00±2.14% hatching
experiment both the males and females were given 71.80±2.00% and survivability 68.50±2.60% in
the 1 st dose but the 2nd injection was given only different months.
to the females after 6 hours. The highest
fertilization rates were 70.10±2.25% in April (dose 527 CHAKRABORTY, B.K. (Directorate of
D 4, 75.00±2.10% in May (dose D 3), 80.00±2.14% Fisheries, Mymensingh); MIAH, M. I.; HABIB,
in June and 79.20±2.65% in July (dose D 3). The M.A.B. & MIRZA, M.J.A. (Dept. of Fisheries,
highest hatching and survivability rates were Bangladesh Agricultural University, Mymensingh).
61.50±3.10 and 55.00±2.13 in April (dose D 4), Embryonic and Larval Development of Indigenous
68.00±3.5 and 63.00±2.20 in May (dose D 3), Sarpunti, Puntius Sarana (Hamilton). Bang. J. Zool.,
71.80±2.00 and 68.50±2.60 in June (dose D 2), and 2007, 35(1), 141-151.
70.25±3.50 and 65.70±2.16 in July (dose D 3)
respectively. The month of June and the dose D 2 (2 The early development stages of an endangered
mg in first injection and 4 mg in second injection) minor carp. Puntius sarana, were described. The
were found to be most effective for induced first cleavage was recorded within 35 minutes
spawning of P. gonionotus. after fertilization and the embryonic rudiments
of developing eggs appeared at 9 hrs and 40
526 BHUIYAN, A.S.; MUSA, A.S.M. & minutes at 27.0 - 27.5°C. The yolk sac was
ISLAM, M.K. (Dept. of Zoology, Rajshahi completely absorbed at 66 hrs of the
University, Rajshahi). Some observations on the development on an attainment of 6.0 mm total
induced spawning of Labeo rohita (Hamilton, length.
1822) by pituitary hormone injection. Bang. J. Life
Sci., 2008, 20(1), 89-94. 528 CHAKRABORTY, B.K. (Govt. of the
People’s Republic of Bangladesh, Directorate of
The study was to determine pituitary hormone Fisheries) & MIRJA, J.A. (Graduate Training
injection doses for optimum breeding performance Institute, Bangladesh Agricultural University,
of Labeo rohita. The experiment was conducted Mymensingh). Induction of spawning, nursing
with five treatments, two replications i.e., each and rearing of endangered bata, Labeo bata
dose was applied to two individual fishes. The (Hamilton-Buchanan) in Bangladesh. Bang. J.
experimental trials were repeated in different Life Sci., 2007, 19(1), 77-87.
months of the breeding season. The female and
male ratio was maintained 1:2. Forty females and Induced breeding of the endangered species, Labeo
eighty males of maintained L. rohita (weighing, bata has been carried out from May to July, 2000.
3.12-4.36 kg) were induced to breed with a series Two hormonal sources were tested to evaluate
st
of PG doses. These were, 1 dose of 1.00 to 5.00 their efficacy on ovulation, fertility and hatching
nd
mg/kg and 2 dose of 2.00 to 10.00 mg/kg body rate of bata, L. bata under controlled conditions.
weight. The performance of each treatment dose Single dose of Ovaprim (0.50 -0.55 ml/kg body
was evaluated in terms of ovulation response, weight) and double doses of PG (an initial dose of
fertilization and hatching rates of eggs. The 2.0 mg/kg body weight and final dose of 4.0-5.5
optimum responses in different months were mg//kg body weight) showed better result in case
achieved with the doses from 6.00 mg/kg to 12.00 of female. Ovaprim also showed better results of
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