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did not vary (P<0.01) between Method 1 and The main purpose of the study was to identify
Method 2 and both gave rise to significantly higher the problems faced by the poultry farmers in
DNA yield than Method 3. The average poultry production as well as to determine the
spectrophotometric ratio for detection of purity of extent of those problems. Data were collected
extracted DNA in Method 1, Method 2 and from randomly selected 88 poultry farmers of
Method 3 were I.492±0.05, 1.362+0.04 and three selected unions of Dumuria upazila under
1.479+0.05 respectively. In terms of purity, these Khulna district through personal interview
methods did not differ significantly (P<0.01). The using an interview schedule during October 07
time required in operational day (Day-2) for the to November 11, 2007. Among the identified
isolation of DNA by Method 1 , Method 2 and 20 problems, the highly common as well as
Method 3 were 7.65, 8.75 and 11.67 hrs highly severe problems were unavailability of
respectively. Method 1 was more efficient and 1 chicks in time, high price of chicks, high price
.10 and 4.22 hrs less time consuming than the of feed, high price of vaccine and medicine,
Method 2 and Method 3 respectively. The cost high production cost and low market price in
(Tk.) of per sample DNA extraction by Method 1, respect of production cost. Among the 5-
Method 2 and Method 3 were 2050.40, 2031.64 typologies of problems, the highly common as
and 2401.31 respectively. Per sample cost of DNA well as highly severe typology of problems was
extraction for Method 1 and Method 2 were almost chick related problems. Growth related and
same but Method 3 was 17.0% and 18.2% more production, marketing and transport related
expensive than Method 1 and Method 2 problems were highly common but they were
respectively. It can be concluded that, Method 1 less and moderately severe respectively. All of
and Method 2 were more or less similar in terms of the respondents belonged to medium problem
quantity, purity and in economic consideration. On confrontation category. Among the selected
the other hand, Method 3 was inferior to Method 1 twelve characteristics of the poultry farmers,
and Method 2 in terms of quantity of DNA, cost only experience in poultry farming, training and
and time required at similar purity. Therefore, extension contact showed a positive significant
Method 1 and Method 2 can be rationally adopted relationship with their problem confrontation.
in our perspective for DNA isolation from blood 484 BHUIYAN, A.A.; FARUQUE, S.;
samples of goat and other species in general. MONIRA, K.N. (Poultry Production Research
Division, Bangladesh Livestock Research Institute,
636.5 : AGRICULTURE : POULTRY Savar, Dhaka) & AZMAL, S.A. (Govt. Poultry
Farm, Comilla). Effects of season and flock size on
483 AHMED, M.B.; BISWAS, S.; MANNAN, cost benefit ratio of broiler production. Bang. J.
M.A. (Agrotechnology Discipline, Khulna Life Sci., 2007, 19 (1), 129-135.
University, Khulna) & AMIN, M.R. (Dept. of
Agricultural Extension Education & Rural Effects of season and flock size on cost benefit
Sociology, Sylhet Agricultural University, ratio of broiler production were studied under
Sylhet). Problem Confrontation of Poultry Poultry Production Research Division, Bangladesh
Farmers of Dumuria Upazila Under Khulna Livestock Research Institute (BLRI) at three
District. South Asian J. Agric., 2007, 2 (1 & 2), different locations, such as, Savar in Dhaka
15-20. District, Baniajuri and Jabra villages in Manikgonj
District and Ramu in Cox's Bazaar District from
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