Page 159 - PARVEEN, S

(80.00%), followed by buck 4 (70.00%) and 2 (CL) absent and present group. Ovaries were then
(67.50+2.50%). After progression of storage
time the motility of sperm decline onto 0.00 to
2.50±2.50% in different bucks after 70 hours.
Sperm motility of fresh and diluted semen was
only the parameter to be evaluated and were
assessed subjectively, which was influenced by
the temperature, diluents and time of storage.
Fresh semen after cooling at 5°C was in an
useable level in a period of less than 24 hours.
Triladyl based diluents achieved greater values
(73.33±1.67%) than Tris based diluents
(70.00±2.89%) just after dilution of semen
before cooling. In relation to time of storage,
sperm motility of diluted semen found in an
useable level after 3 days of storage at 5°C, in
which Triladyl based diluents (46.67-51.67%)
achieved greater values than Tris based diluents
(36.67-46.67%), although the diluted semen
found motile upto day 9 (3.33±3.33%) incase of
Triladyl based diluents and upto day 8
(8.33±1.67%) for Tris based diluents, but the
motility of sperm was not in useable level.
Whether the differences found between the
diluents reflected in the fertility results after AI
is yet unknown and needs to be studied further.
465 PERVAGE, S.; UDDIN, M.J.; BOSTAMI,
A.B.M.R. (Bangladesh Livestock Research Institute,
Savar, Dhaka); KHANDOKER, M.A.M.Y. &
MONDAL, A. (Dept. of Animal Breeding and
Genetics, Bangladesh. Agricultural University,
Mymensingh). Evaluation of Bovine Cumulus
Oocyte-Complexes in view of In Vitro Nuclear
Maturation and Fertilization. Bang. J. Anim. Sci.,
2007, 36 (1 & 2), 16-23.
The present study was undertaken to evaluate the
slaughterhouse cow ovaries and cumulus-oocyte-
complexes (COCs) and to establish the suitable
method of COCs collection in view of in vitro
nuclear maturation and fertilization. Cow ovaries
were categorized as right, left, corpus luteum
152
evaluated on the basis of weight (g), length (cm),
width (cm), total number of follicle on the surface
of each ovaries, number of follicle aspirated and
collected, total number of COCs, normal and
abnormal COCs. It was observed that
comparatively higher number of normal COCs
per ovary was obtained by intact follicle
collection method (3.35) than aspiration method
(1.10). In case of abnormal COCs, the reverse
results were found. The significantly (p<0.01)
higher number of normal COCs (2.56) was found
in CL-absent group than that of CL-present
group. Similarly, an increase of total number of
follicles (5.68), number of follicle aspirated and
collected (4.02) and total number of COCs (3.87)
but decrease in weight (3.19), length (2.22),
width (1.67) and abnormal COCs (1.22). The
higher morphological maturation of COCs was
observed in CL-absent group (75.74%) than CL-
present group (70.83%). The cellular maturation
rate up to metaphase-II of grade A and B COCs
was recorded at 54.75% and 40%, respectively.
The fertilization and further development was
tested. The highest fertilization rate was observed
in grade A (35.16%) than that of grade B
(29.85%) but no further development was recorded
after 24 hours of in vitro culture (IVC).
466 RAHMAN, M.M. & AHMED, A.T.A.
(Dept. of Zoology, Dhaka University, Dhaka).
Studies on Breeding and Larval Development of
Red Bellied Piranha, Pygocentrus Nattereri Kner,
1858 in Bangladesh. Bang. J. Zool., 2007, 35 (2),
193-203.
The pond reared red bellied piranha, Pygocentrus
nattereri Kner, 1858 were spawned through
induced breeding from early June to early October
2005. August and September were the best periods
for spawning. The red piranha was found to be
multiple breeder, i.e. spawned 2-3 times