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Note to paragraph 3. (a): If the test subject eats   (7) The nebulizer is inserted into the hole in
          or drinks something sweet before the screening       the front of the enclosure and an initial concen-
          test, he/she may be unable to taste the weak         tration of saccharin fit test solution is sprayed
          saccharin solution.                                  into the enclosure using the same number of
                                                               squeezes (either 10, 20 or 30 squeezes) based
          (12) If a taste response is elicited, the test sub-  on the number of squeezes required to elicit a
          ject shall be asked to take note of the taste for    taste response as noted during the screening
           reference in the fit test.                          test. A minimum of 10 squeezes is required.

          (13) Correct use of the nebulizer means that ap-     (8) After generating the aerosol, the test subject
          proximately 1 ml of liquid is used at a time in      shall be instructed to perform the exercises in
          the nebulizer body.                                   section I. A. 14. of this appendix.

          (14) The nebulizer shall be thoroughly rinsed        (9) Every 30 seconds the aerosol concentration
          in water, shaken dry, and refilled at least each     shall be replenished using one half the original
          morning and afternoon or at least every four         number of squeezes used initially (e.g., 5, 10 or
          hours.                                               15).


          (b) Saccharin solution aerosol fit test procedure.   (10) The test subject shall indicate to the test
                                                               conductor if at any time during the fit test the
          (1) The test subject may not eat, drink (except      taste of saccharin is detected. If the test subject
          plain water), smoke, or chew gum for 15 min-         does not report tasting the saccharin, the test is
          utes before the test.                                passed.

          (2) The fit test uses the same enclosure de-         (11) If the taste of saccharin is detected, the fit
          scribed in 3. (a) above.                             is deemed unsatisfactory and the test is failed.
                                                               A different respirator shall be tried and the en-
          (3) The test subject shall don the enclosure         tire test procedure is repeated (taste threshold
          while wearing the respirator selected in section     screening and fit testing).
          I. A. of this appendix. The respirator shall be
          properly adjusted and equipped with a par-           (12) Since the nebulizer has a tendency to clog
          ticulate filter(s).                                  during use, the test operator must make peri-
                                                               odic checks of the nebulizer to ensure that it is
          (4) A second DeVilbiss Model 40 Inhalation           not clogged. If clogging is found at the end of
          Medication Nebulizer or equivalent is used to        the test session, the test is invalid.
          spray the fit test solution into the enclosure.
          This nebulizer shall be clearly marked to dis-       4. Bitrex™ (Denatonium Benzoate) Solution
          tinguish it from the screening test solution         Aerosol Qualitative Fit Test Protocol
          nebulizer.
                                                               The Bitrex™ (Denatonium benzoate) solution
          (5) The fit test solution is prepared by adding      aerosol QLFT protocol uses the published sac-
          83 grams of sodium saccharin to 100 ml of            charin test protocol because that protocol is
          warm water.                                          widely accepted. Bitrex is routinely used as a
                                                               taste aversion agent in household liquids
          (6) As before, the test subject shall breathe        which children should not be drinking and is
          through the slightly open mouth with tongue          endorsed by the American Medical Associa-
          extended, and report if he/she tastes the sweet      tion, the National Safety Council, and the
          taste of saccharin.                                  American Association of Poison Control Cen-


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