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(10) When the test subject leaves the chamber, This nebulizer shall be clearly marked to distin-
the subject shall remove the saturated towel guish it from the fit test solution nebulizer.
and return it to the person conducting the test,
so that there is no significant IAA concentration (5) The threshold check solution is prepared by
buildup in the chamber during subsequent dissolving 0.83 gram of sodium saccharin USP
tests. The used towels shall be kept in a self- in 100 ml of warm water. It can be prepared by
sealing plastic bag to keep the test area from putting 1 ml of the fit test solution (see (b)(5)
being contaminated. below) in 100 ml of distilled water.
3. Saccharin Solution Aerosol Protocol (6) To produce the aerosol, the nebulizer bulb
is firmly squeezed so that it collapses com-
The entire screening and testing procedure pletely, then released and allowed to fully ex-
shall be explained to the test subject prior to the pand.
conduct of the screening test.
(7) Ten squeezes are repeated rapidly and then
(a) Taste threshold screening. The saccharin the test subject is asked whether the saccharin
taste threshold screening, performed without can be tasted. If the test subject reports tasting
wearing a respirator, is intended to determine the sweet taste during the ten squeezes, the
whether the individual being tested can detect screening test is completed. The taste threshold
the taste of saccharin. is noted as ten regardless of the number of
squeezes actually completed.
(1) During threshold screening as well as dur-
ing fit testing, subjects shall wear an enclosure (8) If the first response is negative, ten more
about the head and shoulders that is approxi- squeezes are repeated rapidly and the test sub-
mately 12 inches in diameter by 14 inches tall ject is again asked whether the saccharin is
with at least the front portion clear and that al- tasted. If the test subject reports tasting the
lows free movements of the head when a respi- sweet taste during the second ten squeezes, the
rator is worn. An enclosure substantially similar screening test is completed. The taste threshold
to the 3M hood assembly, parts # FT 14 and # is noted as twenty regardless of the number of
FT 15 combined, is adequate. squeezes actually completed.
(2) The test enclosure shall have a 3/4-inch (1.9 (9) If the second response is negative, ten more
cm) hole in front of the test subject’s nose and squeezes are repeated rapidly and the test sub-
mouth area to accommodate the nebulizer ject is again asked whether the saccharin is
nozzle. tasted. If the test subject reports tasting the
sweet taste during the third set of ten squeezes,
(3) The test subject shall don the test enclosure. the screening test is completed. The taste
Throughout the threshold screening test, the threshold is noted as thirty regardless of the
test subject shall breathe through his/her number of squeezes actually completed.
slightly open mouth with tongue extended. The
subject is instructed to report when he/she de- (10) The test conductor will take note of the
tects a sweet taste. number of squeezes required to solicit a taste
response.
(4) Using a DeVilbiss Model 40 Inhalation
Medication Nebulizer or equivalent, the test (11) If the saccharin is not tasted after 30
conductor shall spray the threshold check solu- squeezes (step 10), the test subject is unable to
tion into the enclosure. The nozzle is directed taste saccharin and may not perform the sac-
away from the nose and mouth of the person. charin fit test.
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