Page 274 - AIDSBK23C
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Page 274




               MORTUARY AND FORENSIC LABORATORY PROCEDURES

                       There is minimal risk of exposure to HIV for funeral directors and licensed embalmers
               with use of proper precautions.  Bodies they receive should be handled in designated work areas
               that can be routinely disinfected.  Personnel handling the bodies should wear a protective
               waterproof gown or apron, mask and protective eyewear (or face shield), and disposable gloves.
               Needles and other sharp objects require careful handling with proper disposal into puncture-
               proof containers.  A waterproof, leak proof shroud or body bag should be used for transport of
               bodies.
                       The CDC has made the following specific recommendations for personnel working in
               forensic laboratories:[160]

                       Blood from ALL individuals should be considered potentially infective.  In order to
               supplement other work site precautions, the following precautions are recommended for workers
               in forensic laboratories.

               1.      All specimens of blood should be put in a well-constructed, appropriately labeled
                       container with a secure lid to prevent leaking during transport.  Care should be taken
                       when collecting each specimen to avoid contaminating the outside of the container and
                       the laboratory form accompanying the specimen.

               2.      All persons processing blood specimens should wear gloves.  Masks and protective
                       eyewear or face shields should be worn if mucous-membrane contact with blood is
                       anticipated (e.g., removing tops from vacuum tubes).  Hands should be washed after
                       completion of specimen processing.

               3.      For routine procedures, such as histologic and pathologic studies or microbiological
                       culturing, a biological safety cabinet is not necessary.  However, biological safety
                       cabinets (Class I or II) should be used whenever procedures are conducted that have a
                       high potential for generating droplets.  These include activities such as blending,
                       sonicating, and vigorous mixing.

               4.      Mechanical pipetting devices should be used for manipulating all liquids in the
                       laboratory.  Mouth pipetting must not be done.

               5.      Use of needles and syringes should be limited to situations in which there is no
                       alternative, and the recommendations for preventing injuries with needles outlined under
                       universal precautions should be followed.

               6.      Laboratory work surfaces should be cleaned of visible materials and then decontaminated
                       with an appropriate chemical germicide after a spill of blood, semen, or
                       blood-contaminated body fluid when work activities are completed.
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